Endocrine Abstracts

Introduction: The measurement of free thyroxine (FT4) and free 3,3′,5-triiodothyronine (FT3) in serum by equilibrium dialysis immunoassay methods may suffer from a lack of specificity, therefore measurement by LC/MS/MS has the potential to increase the accuracy of the results. Here we present a sensitive method, employing a simple ultrafiltration sample preparation.Description of methods: 400 uL of serum were processed for 90 minutes using a 3kDa c...